An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Contents:
1. New Methodologies for Multiphase Bioreactors 1: Hydrodynamic and Mass Transfer Characteristics of Multistage Slurry Reactors 2. New Methodologies for Multiphase Bioreactors 2: Image Analysis and Multiphase Bioreactors 3. New Methodologies for Multiphase Bioreactors 3: data Acquisition, Modelling and Control 4. Design and Modelling of Immobilised Biocatalytic Reactors 5. Advances in the Selection and Design of Two-Liquid Phase Biocatalytic Reactors 6. Enzymatic Membrane Reactors 7. Reversed Micellar Bioreaction Systems: Principles and Operation 8. Solid-to-Solid Bioconversions: Batch or Continuous? 9. Solid/Gas Systems, Theory and Applications 10. Biofilm Reactors 11. Pulsing Bioreactors 12. Design of Liquid-Liquid-Solid Fluidises-Bed Bioreactors 13. Flocculation Bioreactors 14. Bioreactor Design For Plant Cell Suspension Cultures 15. Lethal Effects of Bubbles in Animal-Cell Culture 16. A Low-Cost Technology for Entomopathogenic Nematode Large-Scale Production 17. Marine Sponges as Biocatalysts

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Stem Cell Manufacturing discusses the required technologies that enable the transfer of the current laboratory-based practice of stem cell tissue culture to the clinic environment as therapeutics, while concurrently achieving control, reproducibility, automation, validation, and safety of the process and the product. The advent of stem cell research unveiled the therapeutic potential of stem cells and their derivatives and increased the awareness of the public and scientific community for the topic. The successful manufacturing of stem cells and their derivatives is expected to have a positive impact in the society since it will contribute to widen the offer of therapeutic solutions to the patients. Fully defined cellular products can be used to restore the structure and function of damaged tissues and organs and to develop stem cell-based cellular therapies for the treatment of cancer and hematological disorders, autoimmune and other inflammatory diseases and genetic disorders.

Engineering Strategies for Regenerative Medicine considers how engineering strategies can be applied to accelerate advances in regenerative medicine. The book provides relevant and up-to-date content on key topics, including the interdisciplinary integration of different aspects of stem cell biology and technology, diverse technologies, and their applications. By providing massive amounts of data on each individual, recent scientific advances are rapidly accelerating medicine. Cellular, molecular and genetic parameters from biological samples combined with clinical information can now provide valuable data to scientists, clinicians and ultimately patients, leading to the development of precision medicine. Equally noteworthy are the contributions of stem cell biology, bioengineering and tissue engineering that unravel the mechanisms of disease, regeneration and development.

1. Chromatographic Processes.- Modelling Equilibrium and Kinetics in Chromatographic Processes.- Theory of Linear and Nonlinear Chromatography.- Hydrodynamics of Chromatographic Columns.- Cyclic Fixed Bed Sorption Processes for Bioseparations - Design Aspects.- Separations by Continuous Annular Chromatography.- Gradient Elution Chromatography.- Rate Processes in Supercritical Fluid Desorption and Extraction.- 2. Membrane Processes.- Fundamentals of Membrane Separation Processes.- Pressure-Driven Membrane Processes.- Ellectrically-Driven Membrane Processes.- Novel Membranes - Formation and Process Applications.- Cell Harvesting Using Cross-Flow Microfiltration.- Recovery of Intracellular Products.- Pervaporation in Biotechnology.- 3. Affinity Processes.- Affinity Chromatography: Principles and Applications.- Theory of Affinity Chromatography.- Affinity Partitioning.- Prediction of Partition Coefficients for Peptides in Aqueous Two-Phase Systems.- Membrane Affinity Filtration.- 4. Design of Separation Media.- Affinity Chromatography: Design of Biospecific Chromatographic Materials.- Membranes for Bioprocessing: Design Considerations.- 5. Scale-Up, Optimization and Process Integration.- Operating Modes, Scale-up and Optimization of Chromatographic Processes.- Scale-up and Optimization of Membrane Processes.- Simultaneous Reaction and Chromatography.- Membrane bioreactors.- Recombinant Human Tissue-Plasminogen Activator: Biochemistry, Pharmacology, and Process Development.- List of Contributors and Participants.